Tubes should be transported to the sterilization room at close and are to be left in Simple Green solution:
- Put on one glove, transport bio bin from station to the sterilization room counter using single gloved hand.
- With gloved hand, remove lid to ultrasonic, and bio bin
***MAKE SURE THERE IS MIXED ALCONOX AND SIMPLE GREEN SOLUTION IN STERILIZATION ROOM**MAKE SURE AUTOCLAVE HAS WATER***
- Fill ultrasonic with alconox mixture (be careful not to splash!)
- Place two paper towels on counter next to sink (one on top of the other)
- Put on apron, a pair of nitrile gloves, two sleeves (poke thumb holes through sleeves), don a second pair of nitrile gloves over sleeves
- Turn on heat for the ultrasonic
- Place tube container in sink
- Gather necessary cleaning equipment from drawers: pipe cleaners, qtips, allen wrenches (if using multi-piece tubes), toothbrush/steel wire brush etc.
- Remove ultrasonic basket and place on counter on top of paper towels
- Disassemble tubes using allen wrenches (if cleaning multi-piece tubes)
- Scrub all tube parts completely submerged in simple green solution inside container, removing all visible ink
- outside with toothbrush
- inside with pipe cleaners
- Rinse all pieces with water (be careful not to splash!) and place in ultrasonic basket
- Place basket in ultrasonic, making sure all pieces are completely covered with solution
- Dump simple green from tube container down sink drain, rinse the container and put on bio counter
- Cover ultrasonic with cellophane
- Place lid upside down over cellophane and run for 30 minutes
- Remove gloves and all other P.P.E.
- Wash hands, and wait for cycle to complete
- When cycle is complete, put on an apron, a pair of nitrile gloves, two sleeves (poke thumb holes through sleeves), don a second pair of nitrile gloves over sleeves
- Turn off ultrasonic heat and let cool
- Remove basket from ultrasonic and rinse under running water (be careful not to splash!)
- Place tubes on paper towel and leave to dry
- Drain alconox mixture from the ultrasonic
- Remove gloves and all other P.P.E.
- Wash or sanitize hands
- Check water level on autoclave. Fill to appropriate level if necessary
*if tubes are multi-piece follow parts A. If tubes are single-piece follow part B.
Part A:
- Don one glove
- Once tube(s)/pieces have dried, take trays out of autoclave and place on bio counter (be careful not to touch the bio counter with hands!
- With gloved hand, put all tube pieces on autoclave trays
- With gloved hand put autoclave tray into the autoclave
- Remove glove
- Sanitize hands
- Run an autoclave cycle for 45 minutes
- *be sure to log the autoclave cycle (name, date, and time at start – Temperature, pressure, and duration 20 minutes into cycle)
- Don one glove, use one optim wipe to clean bio counter, tube container/lid and ultrasonic
- Once the autoclave cycle is complete and the pieces are dry, reassemble the tubes with a clean allen wrench in a clean area
- In a clean area, seal all tubes into packages with initials and date
- Place packages onto autoclave trays and run another autoclave cycle for 45 minutes
- *be sure to log the autoclave cycle (name, date, and time at start – Temperature, pressure, and duration 20 minutes into cycle)
Part B:
- Pull appropriate number of packages and initial and date each
- Once tube(s)/pieces have dried, don two gloves, take trays out of autoclave and place on bio counter (be careful not to touch the bio counter with hands!
- Seal all tubes into packages
- Place packages onto autoclave trays then place tray(s) into autoclave
- Remove gloves
- Sanitize hands
- Run an autoclave cycle for 45 minutes
- *be sure to log the autoclave cycle (name, date, and time at start – Temperature, pressure, and duration 20 minutes into cycle)
- Don one glove, use one optim wipe to clean bio counter, tube container/lid and ultrasonic
- Remove glove and wash hands
All:
- Once tubes are completely done and packages are dry, put on one glove and transport tubes from the autoclave to their designated storage area
- With ungloved hand grab one paper towel and transfer to gloved hand, with ungloved hand grab alcohol and douse paper towel – wipe down empty bio bin so it looks clean
- With ungloved hand fill tube container with simple green (be careful not to splash!)
- With gloved hand put lid on container then transport to appropriate station
- Remove glove and wash hands